Composite

Part:BBa_K618211:Design

Designed by: Ben Park, Chris Bate, Marc Ammerlaan   Group: iGEM11_Michigan   (2011-09-27)

pLAC ompA-Gli1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE- the sequence as listed here is wrong. Because part K165007 is supplied on a Silver lab vector (Bba_J63009 or V0002) with a modified biobrick prefix, the scar between ompA and Gli-1 is non-standard. The G at position 700 is not there. This has been verified by sequencing. Deleting the G keeps Gli-1 in frame with ompA. Part length is 1224bp.

The sequence was generated automatically and I couldn't figure out how to edit it.

The link below contains the (AFAIK) correct sequence in genbank format:

Media:K618211.gb

Source

parts come from iGEM distribution and from iGEM HQ

References